Student Femtosecond laser based smart procedures for the fabrication of polymeric lab on a chip devicesThursday (25.06.2020) 14:10 - 14:30 Room 3
In this work, a new procedure exploiting the femtosecond laser technology for the rapid prototyping of a Poly(methyl methacrylate) (PMMA) lab on chip device for capturing rare cells is proposed . The fabrication of a serpentine microchannels was performed by direct fs-laser ablation of a PMMA substrate. A new simple, low cost and robust thermally solvent assisted bonding method was also developed for the effective sealing of the microchannel network by covering the microstructured sheet with another plane PMMA layer and bonding them at low temperature and pressure. Leakage tests were performed to assess the reliability of the sealing by injecting liquid into the microchannels. The device prototype was then used for recognizing tumor cells in blood sample. To this purpose the microchannels were functionalized with specific antibodies AntiEpCAM in order to distinguish and recover epithelial tumor cells (MCF7) from a mixed cell suspension with HUVEC thus simulating a heterogeneous blood sample. Functionalization of the microchannels in the sealed microﬂuidic device was performed by a ﬂow-through process: the microchannels were filled with a 3-aminopropyltriethoxylsilane (APTES) ethanol solution and then with glutaraldehyde as cross-linker for the antibodies covalent bonding.
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