Simulation of the tumor ECM using 2PP generated Scaffolds based on (D, L)-Lactide-ɛ-Caprolactone CopolymersThursday (25.06.2020) 14:10 - 14:30 Room 1
Tumorigenesis is correlated with matrix stiffening caused by the synthesis and/or degradation of specific components of the ECM. For analysing the effect of matrix stiffness on changes in the phenotype and genotype of tumor cells, three-dimensional cell-culturing systems (scaffolds) were established. One method for fabrication of scaffolds with defined stiffness values is the 2-Photon Polymerization (2PP).
In our study, scaffolds based on (D, L)-Lactide-ɛ-Caprolactone copolymers with different stiffness values were fabricated by 2PP. These scaffolds were seeded with tumor cells and cellular proliferation and morphology were analyzed. The results show, that we successfully established a highly biocompatible artificial Tumor-ECM with defined stiffness properties by 2PP. We could show that cells colonize the whole scaffold and proliferate inside the scaffold material. The results showed the great potential of the copolymer for simulation of a tumor specific ECM.
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