Laser microfabrication of miniaturized cartridges for extracting immune cells from whole bloodTuesday (23.06.2020) 12:10 - 12:30 Room 1
The aim of this work was to transfer the TACS® technology for the selection of specific immune cells from blood from an existing large volume cartridge into a miniaturized microfluidic prototype.
By analysing the existing FABian® cartridge, a differentiation of the system components could be made and their parameters could be identified by choosing suitable methods.These results were successfully transferred into a multi domain physical systems model in SimulationX®.
The development of the miniaturized cartridge is based on a scaling process in which scaling factors could be determined by analysing the process variables.
Based on these data, a basic design for the microfluidic system was successfully deducted and transferred into a model.
The developed concept was designed via CAD and several prototypes were manufactured. Thereafter a fluidic regimen to operate the microfluidic cartridge was developed and transferred into software. The combination of the prototype cartridge and the microfluidic control was used afterwards to isolate lymphocytes from a buffy coat. The purity of these samples was analysed by flow cytometry. Thus this work shows that the selective isolation of lymphocytes by the microfluidic cartridge could yield to similar purity as the established TACS®-technology
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